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Translation of Synonymous Codons in Family Boxes by Mycoplasma capricolum tRNAs with Unmodified Uridine or Adenosine at the First Anticodon Position

Identifieur interne : 002D35 ( Main/Exploration ); précédent : 002D34; suivant : 002D36

Translation of Synonymous Codons in Family Boxes by Mycoplasma capricolum tRNAs with Unmodified Uridine or Adenosine at the First Anticodon Position

Auteurs : Yuji Inagaki [Japon] ; Atsushi Kojima [Japon] ; Yoshitaka Bessho [Japon] ; Hiroshi Hori [Japon] ; Takeshi Ohama [Japon] ; Syozo Osawa [Japon]

Source :

RBID : ISTEX:93DDAEBB322DEE82CE115A2D290A6B1BDC91AB19

English descriptors

Abstract

Abstract: InMycoplasma capricolum, codon family boxes except for arginine and threonine (CUN leucine, GUN valine, UCN serine, CCN proline, GCN alanine, and GGN glycine) have only a single tRNA species with the anticodon sequence UNN (N is U,C,A or G), the first nucleoside U being modified. Incorporation of the [3H] amino acid into the peptide fraction was examined with theM. capricolumcell-free translation system. Synthetic mRNA containing each of the respective amino acid codons in the coding frame was subjected to translation. The tRNAUNNtranslated all the family box codons with similar, if not equal, efficiencies. InM. capricolum, there are two species of threonine tRNA, tRNAUGUThrand tRNAAGUThr, the first nucleoside U or A being unmodified. The tRNAUGUThrspecies translates codons ACA, ACG and ACU efficiently, and ACC only poorly. In contrast, the tRNAAGUThrspecies translates codons ACU, ACC and ACG efficiently and ACA poorly.

Url:
DOI: 10.1006/jmbi.1995.0450


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<term>Mycoplasma</term>
<term>Mycoplasma capricolum</term>
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<term>Natl acad</term>
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<term>Pairing</term>
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<term>Synonymous codons</term>
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<div type="abstract" xml:lang="en">Abstract: InMycoplasma capricolum, codon family boxes except for arginine and threonine (CUN leucine, GUN valine, UCN serine, CCN proline, GCN alanine, and GGN glycine) have only a single tRNA species with the anticodon sequence UNN (N is U,C,A or G), the first nucleoside U being modified. Incorporation of the [3H] amino acid into the peptide fraction was examined with theM. capricolumcell-free translation system. Synthetic mRNA containing each of the respective amino acid codons in the coding frame was subjected to translation. The tRNAUNNtranslated all the family box codons with similar, if not equal, efficiencies. InM. capricolum, there are two species of threonine tRNA, tRNAUGUThrand tRNAAGUThr, the first nucleoside U or A being unmodified. The tRNAUGUThrspecies translates codons ACA, ACG and ACU efficiently, and ACC only poorly. In contrast, the tRNAAGUThrspecies translates codons ACU, ACC and ACG efficiently and ACA poorly.</div>
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